Primer Premier 5 lets you design cross species, as well as pathogenic- and allele-specific primers at the click of a button. It will also design PCR, sequencing and hybridization primers automatically. The program supports degenerate, multiplex/nested primer design, sequence translation and restriction enzyme analysis. Primer 5 is the first program to fully integrate multiple sequence alignment (ClustalW) with primer design to facilitate the design of primers that can be used for cross species, pathogenic or allele specific research. Note: System requirements: Mac OS 9.
Notes Tucows, Inc has graciously donated a copy of this software to the Internet Archive's Tucows Software Archive for long term preservation and access. Please check the for all current versions of the software.
Primer Premier is a Home & Education software developed by PREMIER Biosoft International. After our trial and test, the software is proved to be official, secure and free. Here is the official description for Primer Premier: EditByBrothersoft: Extensive Primer Design Support Primer Premier is a comprehensive primer design tool for PCR, hybridization, cross species, sequencing and allele specific polymerase chain reaction. Integrated Alignment Primer Premier fully integrates multiple sequence alignment for consensus or species specific PCR primer design using the well proven, widely used. Degenerate and Multiplex Primer Design With Primer Premier you can start with an amino sequence, reverse translate and design primers in regions of low degeneracy automatically. Primer Premier designs highly specific primers for multiplex experiments and analyzes them for homologies. Sequence Along with PCR primer design, use Primer Premier to analyze your templates using an extensive built-in database of common enzymes and motifs.
You can even add, delete or edit and motif information to customize the program for your experiments. Comprehensive Reports View and print comprehensive reports on hairpin, dimer, false priming, optical activity and internal stability of your designed primers. Versatile Primer Analysis Primer Premier lets you analyze pre-designed and published primers.
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You can also design primers for site directed mutagenesis. Features New in Primer Premier Cross species primers: Designs primers for amplifying sequences from multiple species. (For Real Time qPCR cross species primers, try AlleleID) ClustalW multiple sequence alignment: Aligns multiple sequences using ClustalW 1.7 algorithm.
Minority consensus: Generates consensus sequence using degenerate bases. Alignment editor: Manually insert/delete/move gaps in an alignment.
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Constitutional law of india by j n pandey pdf files. File Format Support: Now supports various file formats like GenBank, NBRF/PIR, EMBL/SWISSPROT and FASTA. Sequence Editor Long PCR: Handles sequences up to 50 KB. Alternate views: View sequence as sense, anti-sense, or double stranded.
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